| First Author | Mackman N | Year | 1992 |
| Journal | Arterioscler Thromb | Volume | 12 |
| Issue | 4 | Pages | 474-83 |
| PubMed ID | 1348427 | Mgi Jnum | J:1127 |
| Mgi Id | MGI:49659 | Doi | 10.1161/01.atv.12.4.474 |
| Citation | Mackman N, et al. (1992) Structure of the murine tissue factor gene. Chromosome location and conservation of regulatory elements in the promoter. Arterioscler Thromb 12(4):474-83 |
| abstractText | Tissue factor (TF) is a transmembrane glycoprotein that mediates cellular initiation of the coagulation serine protease cascades. Moreover, expression of TF in human atherosclerotic plaques is likely to play a significant role in the thrombotic complications associated with plaque rupture. In this study the complete murine TF gene, Cf-3, was isolated from mouse NIH 3T3 cells and was found to consist of six exons spanning about 11 kilobase pairs (kbp) of DNA. A major transcriptional start site was located 24 bp downstream of a TATA box. Cf-3 was mapped to chromosome 3 by analysis of an intersubspecies test cross. Conserved transcription factor-binding sites were identified by comparison of 5' flanking regions of the murine and human TF genes. A region of the TF promoter required for constitutive expression exhibited 85% identity in DNA sequence and included two conserved binding sites for Sp1. Furthermore, two AP-1 sites and an NF-kappa B site were conserved in a 56-bp region necessary for transcriptional activation in response to bacterial lipopolysaccharide. These highly conserved regions of the TF promoter, which contain several binding sites for well-characterized transcription factors, are likely to be functionally important in the complex pattern of TF gene expression observed in a variety of cell types. |
