| First Author | Abboud SL | Year | 1992 |
| Journal | Br J Haematol | Volume | 80 |
| Issue | 4 | Pages | 452-7 |
| PubMed ID | 1581229 | Mgi Jnum | J:467 |
| Mgi Id | MGI:49004 | Doi | 10.1111/j.1365-2141.1992.tb04557.x |
| Citation | Abboud SL (1992) Epidermal growth factor stimulates macrophage colony-stimulating factor (M-CSF) mRNA expression and M-CSF release in cultured murine stromal cells. Br J Haematol 80(4):452-7 |
| abstractText | Macrophage colony-stimulating factor (M-CSF) released by stromal cells of the bone marrow microenvironment plays a crucial role in the growth and proliferation of mononuclear cells. Several peptide mitogens including interleukin-1, tumour necrosis factor, platelet-derived growth factor and fibroblast growth factor stimulate the release of M-CSF and may be important in mediating the haematopoietic response to inflammation. Epidermal growth factor (EGF), released from platelets during aggregation, is mitogenic for a variety of cell types and may cause the release of certain cytokines. In this study we used the TC-1 murine stromal cells which constitutively secrete M-CSF as a model to study the regulation of M-CSF in response to EGF. EGF markedly stimulated the steady state expression of M-CSF mRNA with a peak effect observed at 3 h. This was associated with the release of M-CSF protein as determined by radioimmunoassay. EGF also stimulated DNA synthesis in a concentration dependent manner. Although TC-1 cells express GM-CSF mRNA, this was not induced by EGF. These findings suggest that EGF is a key regulatory molecule for M-CSF and may indirectly effect haematopoiesis via the release of M-CSF from stromal cells. |
