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Publication : Molecular characterization of a mouse Y chromosomal repetitive sequence that detects transcripts in the testis.

First Author  Prado VF Year  1992
Journal  Cytogenet Cell Genet Volume  61
Issue  2 Pages  87-90
PubMed ID  1395731 Mgi Jnum  J:2905
Mgi Id  MGI:51425 Doi  10.1159/000133375
Citation  Prado VF, et al. (1992) Molecular characterization of a mouse Y chromosomal repetitive sequence that detects transcripts in the testis. Cytogenet Cell Genet 61(2):87-90
abstractText  145SC5 is a Y chromosomal repetitive sequence isolated from a BALB/c mouse and is unique in that it detects poly(A)-containing transcripts in the testis. We determined nucleotide sequences of 145SC5 and a related cDNA clone designated PC11 and compared their sequences to that of pYMT2/B, another related cDNA clone. 145SC5 and PC11 are almost identical (97%), while PC11 and pYMT2/B share 84% identity. In situ hybridization and Southern blot analysis with XXSxr male mice demonstrated that 145SC5-related sequences were distributed over the entire length of the Y chromosome including the short arm. Two types of Y chromosome are present in inbred mouse strains: the Mus musculus musculus type and M.m. domesticus type. Among 26 inbred strains surveyed, 145SC5 detected polymorphims only in the M.m. domesticus Y chromosome.
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