| First Author | Schiff R | Year | 1993 |
| Journal | Endocrinology | Volume | 133 |
| Issue | 2 | Pages | 529-37 |
| PubMed ID | 8344198 | Mgi Jnum | J:14027 |
| Mgi Id | MGI:62204 | Doi | 10.1210/endo.133.2.8344198 |
| Citation | Schiff R, et al. (1993) Expression and cellular localization of uterine side-chain cleavage cytochrome P450 messenger ribonucleic acid during early pregnancy in mice. Endocrinology 133(2):529-37 |
| abstractText | Very little is known about steroidogenic capacities in the uterus during the early stages of pregnancy in rodents. Cholesterol side-chain cleavage cytochrome P450 (P450scc) is the enzyme catalizing the first and key regulatory reaction controlling the production of steroid hormones. Using a cRNA probe, we made use of in situ hybridization analysis to evaluate the spatial and temporal patterns of P450scc mRNA expression in the mouse uterus until midgestation. Unexpectedly, we found that upon implantation on day 4.5, maternal cells of both decidua capsularis and decidua basalis expressed P450scc mRNA. Only later, and no earlier than day 6.5 of gestation, were high levels of P450scc mRNA also detected in the trophoblast giant cells surrounding the embryonal cavity. Analysis of pseudopregnant mice revealed that the induction of P450scc mRNA can be coupled to the decidual reaction evoked by intrauterine injection of mineral oil. These results, therefore, unambiguously confirmed the capacity of the decidualized cells of maternal origin to express P450scc mRNA and, thus, ruled out any direct role of the blastocyst involvement in P450scc induction. The dual localization of P450scc mRNA in maternal and trophoblast cells, expressing this cytochrome earlier than the previously suspected onset of uterine steroidogenesis, suggests an unexpected role for steroid hormones locally produced at the site of implantation and the surrounding milieu of the embryo during the first half of pregnancy. |
