| First Author | García-Sanz JA | Year | 1993 |
| Journal | Eur J Immunol | Volume | 23 |
| Issue | 8 | Pages | 1877-83 |
| PubMed ID | 8344349 | Mgi Jnum | J:14800 |
| Mgi Id | MGI:62961 | Doi | 10.1002/eji.1830230822 |
| Citation | Garcia-Sanz JA, et al. (1993) Regulation of perforin gene expression in a T cell hybrid with inducible cytolytic activity. Eur J Immunol 23(8):1877-83 |
| abstractText | A mouse x rat T cell hybrid (PC60) that does not require interleukin (IL)-2 for proliferation, was used as a model to study regulation of perforin gene expression. Perforin mRNA is barely detectable in non-induced PC60 cells; however, a 30-fold induction is observed after stimulation with IL-1 alone. Peak perforin mRNA levels were reached after 10 h of induction with IL-1, and these levels were maintained for as long as the stimulus was present. IL-2 by itself has no detectable effect. However, in combination with IL-1 it shows the same induction kinetics as IL-1 alone for the first 10 h, subsequently there is synergism (100-fold induction) between IL-1 and IL-2. The induction response was mainly due to increased transcriptional rates of the perforin gene, and require newly synthesized proteins. The half-life of perforin mRNA in this system is about 5 h. In addition, we confirm the existence of two types of mouse perforin mRNA that differ in their 5' untranslated regions, and show evidence that both mRNA are translated in vivo with similar efficiencies. |
