| First Author | Schleef M | Year | 1993 |
| Journal | Genomics | Volume | 17 |
| Issue | 2 | Pages | 519-21 |
| PubMed ID | 8406508 | Mgi Jnum | J:13592 |
| Mgi Id | MGI:61778 | Doi | 10.1006/geno.1993.1361 |
| Citation | Schleef M, et al. (1993) Chromosomal localization and genomic cloning of the mouse alpha-tropomyosin gene Tpm-1. Genomics 17(2):519-21 |
| abstractText | In vertebrates, the alpha-tropomyosin gene, Tpm-1, codes for at least 9 tropomyosin isoforms that are expressed by tissue-specific alternative splicing. Using interspecies backcrosses, we have localized Tpm-1 on mouse chromosome 9, cen-Cyp1a2-Tpm-1-Mod-1-Mylc-Scn5a, near the d-se region. The restriction fragment length variant used for chromosomal assignment, as well as other restriction fragments hybridizing to a 3'-specific alpha-tropomyosin cDNA probe in genomic Southern blots, was investigated by cloning 17.5-kb of Tpm-1. The same restriction patterns were observed, proving the identity of the mapped and the cloned gene. The identity was supported by sequencing the 3' end of the gene. |
