| First Author | Warner CM | Year | 1993 |
| Journal | J Reprod Immunol | Volume | 24 |
| Issue | 2 | Pages | 111-21 |
| PubMed ID | 8229991 | Mgi Jnum | J:14353 |
| Mgi Id | MGI:62523 | Doi | 10.1016/0165-0378(93)90014-9 |
| Citation | Warner CM, et al. (1993) Induction of embryonic major histocompatibility complex antigen expression by gamma-IFN. J Reprod Immunol 24(2):111-21 |
| abstractText | Preimplantation mouse embryos were incubated in vitro with mouse recombinant gamma-interferon (IFN). The effect of the gamma-IFN on major histocompatibility complex (MHC) class I antigen expression was tested using an ELISA procedure. It was found that there is a doubling of Db antigens and a tripling of Qa-2 antigens on C57BL/6 mouse embryos cultured from the 8-cell stage for 24 h in the presence of 10(5) units/ml gamma-IFN. The effect of gamma-IFN on the rate of preimplantation embryonic development was tested by culturing 2-cell embryos for 48 h and 8-cell embryos for 24 h in the presence of varying concentrations of gamma-IFN up to 10(6) units/ml. Two methods were used to assess the cell number per embryo after the culture period: incorporation of [3H]thymidine into DNA, and direct counting of nuclei in fixed and stained embryos. Both methods showed that treatment with gamma-IFN increases the rate of development of preimplantation mouse embryos. Since rate of preimplantation embryonic development is genetically controlled by the Ped gene, it is suggested that gamma-IFN has a direct effect on the Ped gene phenotype of preimplantation mouse embryos. |
