| First Author | Alkema MJ | Year | 1993 |
| Journal | Hum Mol Genet | Volume | 2 |
| Issue | 10 | Pages | 1597-603 |
| PubMed ID | 8268912 | Mgi Jnum | J:15240 |
| Mgi Id | MGI:63369 | Doi | 10.1093/hmg/2.10.1597 |
| Citation | Alkema MJ, et al. (1993) Characterization and chromosomal localization of the human proto-oncogene BMI-1. Hum Mol Genet 2(10):1597-603 |
| abstractText | The proto-oncogene bmi-1 is frequently activated by Moloney murine leukemia proviral insertions in E mu-myc transgenic mice1,2. Using a mouse bmi-1 cDNA probe a transcript of 3.3 kb was detected on Northern blots of human Burkitt's lymphoma cell lines. We have isolated and sequenced cDNA clones from a human erythroleukemia cell line (K562) derived cDNA library, using different mouse bmi-1 cDNA fragments as a probe. Analysis of genomic BMI-1 sequences reveals a gene structure which is very similar to that of the mouse, consisting of at least 10 exons. The human cDNA is 3203 bp in length and shows 86% identity to the mouse nucleotide sequence. The open reading frame encodes a protein of 326 amino acids which shares 98% identity to the amino acid sequence of mouse bmi-1 protein. In vitro translation experiments show that human cDNA derived RNA translates into a protein with a mobility of 44-46 kD on SDS polyacrylamide gels. Fluorescence in situ hybridization (FISH) on metaphase chromosome spreads located the human BMI-1 gene to the short arm of chromosome 10 (10p13), a region known to be involved in translocations in various leukemias. |
