| First Author | Fundele R | Year | 1994 |
| Journal | Dev Biol | Volume | 161 |
| Issue | 1 | Pages | 30-6 |
| PubMed ID | 8293880 | Mgi Jnum | J:16413 |
| Mgi Id | MGI:64494 | Doi | 10.1006/dbio.1994.1004 |
| Citation | Fundele R, et al. (1994) Early skeletal muscle development proceeds normally in parthenogenetic mouse embryos. Dev Biol 161(1):30-6 |
| abstractText | In mouse chimeras with parthenogenetic cell contribution, the skeletal musculature appears to be largely devoid of parthenogenetically derived cells. To analyze the appearance and early distribution of myotomal cells in parthenotes, we determined the expression of the muscle-specific transcription factors myogenin, MYF-5, and MYF-6 by in situ hybridization in somites of Day 10 and 11 embryos. Here, we report that these myogenic regulatory proteins are expressed in parthenogenetic animals together with desmin, one of the early muscle-specific structural proteins. We also show that parthenogenetic cells contribute equally to dermatome, sclerotome, and myotome in Day 10 and 11 chimeras. These results suggest that early myotomal cells expressing the myogenic control proteins develop and allocate normally in parthenogenetic embryos and in parthenogenetic<==>normal chimeras. The underrepresentation in older chimeras may therefore be due to selective elimination. These data also argue against imprinting of the myogenic factor genes myogenin, Myf-5, and Myf-6. |
