| First Author | Tam SW | Year | 1994 |
| Journal | Gene | Volume | 139 |
| Issue | 2 | Pages | 171-6 |
| PubMed ID | 7509303 | Mgi Jnum | J:17003 |
| Mgi Id | MGI:65059 | Doi | 10.1016/0378-1119(94)90751-x |
| Citation | Tam SW, et al. (1994) Human cathepsin B-encoding cDNAs: sequence variations in the 3'-untranslated region. Gene 139(2):171-6 |
| abstractText | We have isolated two cathepsin B (CTSB)-encoding cDNAs, hCBF1 and hCBF2, from a normal human embryonic fibroblast library. These clones demonstrate 98% identity to overlapping regions of published human hepatoma and kidney CTSB cDNAs, but show some interesting differences from the published sequences in the 3'-untranslated region (3'-UTR). Both hCBF1 and hCBF2 contain a 10-bp insertion in the 3'-UTR that may permit formation of a highly stable stem-loop structure not present in mRNAs without this insertion. Our hCBF1 cDNA also contains a 1019-bp extension of the 3'-UTR sequence that resembles the long 3'-UTR reported for murine CTSB cDNAs. Probes unique to this 3'-UTR extension hybridize to 4.0- and 1.7-kb CTSB RNAs on Northern blots, but not to the major 2.2-kb mRNA transcript. Our data reveal variations in normal human CTSB transcripts that result from differences in the length of the 3'-UTR, as well as the presence or absence of a stem-loop stabilizing sequence. |
