| First Author | Yoshida A | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 198 |
| Issue | 3 | Pages | 857-61 |
| PubMed ID | 7906942 | Mgi Jnum | J:16881 |
| Mgi Id | MGI:64940 | Doi | 10.1006/bbrc.1994.1122 |
| Citation | Yoshida A, et al. (1994) IFN-gamma induces IL-12 mRNA expression by a murine macrophage cell line, J774. Biochem Biophys Res Commun 198(3):857-61 |
| abstractText | Interleukin (IL)-12 is a logical candidate for participation in the differentiation of T helper (Th) 1 cells. IL-12 is produced by macrophages and B cells and induces the production of interferon (IFN)-gamma from Th1 cells and NK cells. In this study, we show that the IFN-gamma itself is capable of inducing IL-12 mRNA expression in murine macrophage cell line J774. The mRNA was apparent by 12h after IFN-gamma treatment, although maximal induction required 36 to 48h. Furthermore, we investigated the signal transduction mechanism responsible for the mRNA expression. The IFN-gamma-inducible IL-12 mRNA was blocked by two protein tyrosine kinase (PTK) inhibitors, genistein and herbimycin A, suggesting that PTK is involved in the IFN-gamma-inducible IL-12 mRNA expression. |
