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Publication : Cloning and expression of a human glutamate transporter.

First Author  Kawakami H Year  1994
Journal  Biochem Biophys Res Commun Volume  199
Issue  1 Pages  171-6
PubMed ID  8123008 Mgi Jnum  J:17039
Mgi Id  MGI:65094 Doi  10.1006/bbrc.1994.1210
Citation  Kawakami H, et al. (1994) Cloning and expression of a human glutamate transporter. Biochem Biophys Res Commun 199(1):171-6
abstractText  A complementary DNA clone was isolated from human brain that encodes a glutamate transporter. Injection of RNA transcribed from this cDNA into Xenopus oocytes resulted in expression of a transport activity with a high affinity for glutamate (Km = 78.4 microM) and a dependency on external Na+. The cDNA sequence predicts a protein of 542 amino acids that is highly homologous to the congeneric proteins from rat and mouse brains. RNA blotting analyses revealed the expression both in the brain and in peripheral tissues.
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