| First Author | Doherty AS | Year | 1994 |
| Journal | Mol Reprod Dev | Volume | 37 |
| Issue | 1 | Pages | 21-6 |
| PubMed ID | 8129927 | Mgi Jnum | J:16135 |
| Mgi Id | MGI:64225 | Doi | 10.1002/mrd.1080370104 |
| Citation | Doherty AS, et al. (1994) Temporal pattern of IGF-I expression during mouse preimplantation embryogenesis. Mol Reprod Dev 37(1):21-6 |
| abstractText | Although mouse preimplantation embryos express transcripts for the insulin-like growth factor-I receptor (IGF-IR), IGF-I transcripts were not detected by reverse transcription-PCR (RT-PCR) at any stage of preimplantation development in a previous study (Rappolee et al., Genes Dev 6:939-952, 1992). We report that IGF-I transcripts are detected in the preimplantation embryo by RT-PCR and describe the temporal pattern of expression of this transcript from the oocyte to blastocyst stages; the level declines from the oocyte to 8-cell embryo and then increases from the 8-cell to blastocyst stages. An explanation is offered to account for the differences in detecting the IGF-I transcript, and the results are discussed in the context of an autocrine/paracrine IGF-I circuit in the preimplantation mouse embryo. |
