| First Author | Lee G | Year | 1994 |
| Journal | Mol Cell Biol | Volume | 14 |
| Issue | 7 | Pages | 4579-87 |
| PubMed ID | 8007962 | Mgi Jnum | J:18887 |
| Mgi Id | MGI:67109 | Doi | 10.1128/mcb.14.7.4579 |
| Citation | Lee G, et al. (1994) Dual modes of control of c-fos mRNA induction by intracellular calcium in T cells. Mol Cell Biol 14(7):4579-87 |
| abstractText | Cytoplasmic calcium is a nearly universal second messenger in eukaryotes. In many cell types, elevated intracellular calcium interacts synergistically with inducers of protein kinase C to elicit activation of complete biological programs normally induced by extracellular signals. In T cells, elevated cytoplasmic calcium is a critical mediator of activation in response to stimulation of the antigen receptor, and in some T-cell lines, treatment with a combination of calcium ionophore and protein kinase C activator mimics authentic antigen treatment. The synergistic interaction of calcium and protein kinase C in T cells is also observed at the level of gene expression. Here we examine the molecular mechanisms through which these agents exert synergistic control over the expression of the c-fos proto-oncogene in a T-cell hybridoma. We find that the principal effect of calcium is on the elongation of c-fos transcripts. This step constitutes the major control of c-fos mRNA accumulation in these cells. In addition, calcium regulates the initiation of c-fos transcription. This effect requires the serum response element of the c-fos gene and an additional sequence immediately 3' to this element. Thus, calcium regulates c-fos expression through at least two distinct molecular pathways. |
