| First Author | Ishizaki K | Year | 1994 |
| Journal | Int J Cancer | Volume | 58 |
| Issue | 2 | Pages | 254-7 |
| PubMed ID | 8026887 | Mgi Jnum | J:19174 |
| Mgi Id | MGI:67369 | Doi | 10.1002/ijc.2910580218 |
| Citation | Ishizaki K, et al. (1994) Increased UV-induced SCEs but normal repair of DNA damage in p53-deficient mouse cells. Int J Cancer 58(2):254-7 |
| abstractText | UV-induced sister chromatid exchanges (SCEs) in p53-deficient mouse cells were studied to obtain more evidence regarding the involvement of p53 protein in the DNA repair pathway as a checkpoint protein. After 5 J/m2 UV irradiation, mutant-type homozygous cells for p53-deficiency showed the same number of SCEs as the heterozygous and wild-type homozygous cells. In the heterozygous and wild-type homozygous cells, no further increase of SCEs was observed after 10 J/m2 UV irradiation. In contrast, in mutant-type homozygous cells about twice as many SCEs were induced by 10 J/m2 UV as by 5 J/m2 UV. In mutant-type homozygous cells, fractions of S-phase cells decreased just after 10 J/m2 UV irradiation, but recovered to higher than control levels within a short time, while in heterozygous and wild-type homozygous cells, the decrease in S-phase cells was prolonged by more than 6 hr and no increase above control levels was observed. Although no difference in UV sensitivity and repair of UV-induced DNA damage was found among the 3 genotypes, which were determined by the relative colony-forming ability after UV irradiation and removal of thymine dimers and (6-4) photoproducts from cellular DNA, our data strongly suggest an impaired checkpoint function in p53-deficient cells when DNA is damaged. |
