| First Author | Stone B | Year | 1994 |
| Journal | Nucleic Acids Res | Volume | 22 |
| Issue | 13 | Pages | 2612-8 |
| PubMed ID | 7518916 | Mgi Jnum | J:53997 |
| Mgi Id | MGI:1333723 | Doi | 10.1093/nar/22.13.2612 |
| Citation | Stone B, et al. (1994) Targeted RNA fingerprinting: the cloning of differentially-expressed cDNA fragments enriched for members of the zinc finger gene family. Nucleic Acids Res 22(13):2612-8 |
| abstractText | We have developed and applied a modification of an 'RNA Fingerprinting' protocol previously published by Welsh and McClelland (Nucleic Acids Research 19: 5275-5279 1991) such that cDNA fragments which are both differentially-expressed and enriched for members of a specific gene family can readily be identified. cDNA fragments were amplified with an arbitrary primer initially used in the reverse transcription reaction in combination with a member of a primer set which corresponded to a conserved region within a specific gene family. This technique was used to isolate cDNAs encoding a recently described protein kinase as well as an unknown gene that contained a zinc finger. Several other known genes that contained a zinc finger domain and that were differentially-expressed were also isolated. |
