| First Author | Zhang-Keck ZY | Year | 1994 |
| Journal | Biochem J | Volume | 301 ( Pt 3) |
| Pages | 835-45 | PubMed ID | 8053909 |
| Mgi Jnum | J:19685 | Mgi Id | MGI:68833 |
| Doi | 10.1042/bj3010835 | Citation | Zhang-Keck ZY, et al. (1994) Genomic organization and chromosomal localization of the mouse synexin gene. Biochem J 301(Pt 3):835-45 |
| abstractText | We have isolated and characterized the gene encoding mouse synexin, which consists of 14 exons and spans approximately 30 kbp of genomic DNA. The protein's unique N-terminal domain is encoded by six exons, and the C-terminal tetrad repeat, the site of the membrane-fusion and ion-channel domain, is encoded by seven exons. The first exon encodes the 5'-untranslated region. Analysis of synexin-gene expression in different mouse tissues shows that mRNA with exon 6 is only present in brain, heart and skeletal muscle. mRNA lacking exon 6 is expressed in all tissues we have examined. The initiation site for transcription was determined by primer-extension analysis and S1 nuclease mapping. Sequence analysis of the 1.3 kb 5'-flanking region revealed that the promoter has a TATA box located at position -25 and a number of potential promoter and regulatory elements. A CCAAT motif was not observed but CCATT is located in an appropriate position for the CCAAT motif upstream from the transcription-initiation start site. In addition, the 5'-flanking region contains two sets of palindromic sequences. Finally, we have determined that the functional synexin gene (Anx7) is located on mouse chromosome 14 and that a pseudogene (Anx7-ps1) is located on chromosome 10. |
