| First Author | Shackelford R | Year | 1995 |
| Journal | J Immunol | Volume | 154 |
| Issue | 3 | Pages | 1374-82 |
| PubMed ID | 7822804 | Mgi Jnum | J:23023 |
| Mgi Id | MGI:70655 | Doi | 10.4049/jimmunol.154.3.1374 |
| Citation | Shackelford R, et al. (1995) IFN-gamma and lipopolysaccharide induce DNA binding of transcription factor PU.1 in murine tissue macrophages. J Immunol 154(3):1374-82 |
| abstractText | The transcription factor PU.1, the Spi-1 oncogene product, is found principally in B cells and macrophages. PU.1 binds to a purine-rich sequence of DNA with a core consensus sequence of 5'-GAGGAA-3'. By using an electrophoretic mobility shift assay, we have been able to identify PU.1 in nuclear extracts of murine tissue macrophages. When macrophages are stimulated with IFN-gamma, an increase in PU.1 binding is observed. This increase is gradual, peaking at 6 to 9 h and decaying to basal levels 24 h after stimulation. The increase in PU.1 binding is unaffected by inhibiting protein synthesis, but is attenuated by either an inhibitor of the NA+/H+ antiporter or elevations in cAMP. In addition, we have found that low doses (1 ng/ml) of bacterial LPS induce PU.1 binding. |
