| First Author | Hunter KW | Year | 1994 |
| Journal | Mamm Genome | Volume | 5 |
| Issue | 10 | Pages | 597-607 |
| PubMed ID | 7849394 | Mgi Jnum | J:20963 |
| Mgi Id | MGI:69017 | Doi | 10.1007/BF00411453 |
| Citation | Hunter KW, et al. (1994) Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): generation of a 5-cM, > 5 megabase contig on mouse chromosome 1. Mamm Genome 5(10):597-607 |
| abstractText | We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, > 5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order to the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ-gld and M. spretus. Three probes were mapped on a [(C3H/HeJ-gld x M. spretus) x C3H/HeJ-gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region. |
