| First Author | Kaushik N | Year | 1994 |
| Journal | Mamm Genome | Volume | 5 |
| Issue | 11 | Pages | 688-95 |
| PubMed ID | 7873878 | Mgi Jnum | J:21511 |
| Mgi Id | MGI:69479 | Doi | 10.1007/BF00426074 |
| Citation | Kaushik N, et al. (1994) Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR. Mamm Genome 5(11):688-95 |
| abstractText | We describe a novel, PCR-based technique termed REVEAL-PCR for examining the inheritance of intracisternal A-type particles (IAP). Amplifications use an unlabeled primer to SINE repeats and a radiolabeled primer to the IAP long terminal repeat; labeled products, which can be resolved on sequencing gels, are formed when IAPs lie in proximity to SINEs. With this technique we have identified a total of 124 polymorphisms in the BXH and CXS recombinant inbred strains. We suggest that this method will be equally applicable for examining other gene families present at around a thousand copies per genome. |
