| First Author | Hu Q | Year | 1995 |
| Journal | Science | Volume | 268 |
| Issue | 5207 | Pages | 100-2 |
| PubMed ID | 7701328 | Mgi Jnum | J:24206 |
| Mgi Id | MGI:71955 | Doi | 10.1126/science.7701328 |
| Citation | Hu Q, et al. (1995) Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase. Science 268(5207):100-2 |
| abstractText | Phosphatidylinositol (Pl)-3 kinase is one of many enzymes stimulated by growth factors. A constitutively activated mutant, p110, that functions independently of growth factor stimulation was constructed to determine the specific responses regulated by Pl-3 kinase. The p110 protein exhibited high specific activity as a Pl-3 kinase and as a protein kinase. Expression of p110 in NIH 3T3 cells induced transcription from the fos promoter. Co-expression of dominant negative Ras blocked this response. When expressed in Xenopus laevis oocytes, p110 increased the amount of guanosine 5'-triphosphate-bound Ras, caused activation of the Ras effector Raf-1, and induced Ras-dependent oocyte maturation. These findings show that Pl-3 kinase can stimulate diverse Ras-dependent cellular processes, including oocyte maturation and fos transcription. |
