| First Author | Granneman JG | Year | 1995 |
| Journal | Am J Physiol | Volume | 268 |
| Issue | 4 Pt 1 | Pages | C1040-4 |
| PubMed ID | 7733225 | Mgi Jnum | J:24492 |
| Mgi Id | MGI:72231 | Doi | 10.1152/ajpcell.1995.268.4.C1040 |
| Citation | Granneman JG, et al. (1995) Regulation of mouse beta 3-adrenergic receptor gene expression and mRNA splice variants in adipocytes. Am J Physiol 268(4 Pt 1):C1040-4 |
| abstractText | This study examined the regulation of murine beta 3-receptor mRNA and determined whether the recently described mRNA splice variants are differentially regulated by agents that alter total beta 3-receptor mRNA levels. In vivo treatment of mice with the beta 3-receptor agonist BRL-26830 reduced total beta 3-transcripts by 64% in white adipose tissue but did not alter the mRNA splicing pattern. Further analysis in cultured 3T3-F442A adipocytes showed that isoproterenol, dexamethasone, or phorbol 12-myristate 13-acetate also greatly reduced beta 3-receptor mRNA levels without selectively altering poly-U-containing transcripts. Blockade of transcription with actinomycin D produced a rapid loss of beta 3-receptor mRNA, which was prevented by blockade of mRNA translation with cycloheximide. However, neither actinomycin D nor cycloheximide altered the splicing pattern of beta 3-receptor mRNA. Analysis of transcription rate by nuclear run-off assay indicated that 8-bromoadenosine 3',5'-cyclic monophosphate and phorbol 12-myristate 13-acetate reduce beta 3-receptor gene transcription and that suppression of transcription is sufficient to account for the reduction in beta 3-receptor mRNA levels by these agents. |
