| First Author | Manavalan P | Year | 1995 |
| Journal | FEBS Lett | Volume | 366 |
| Issue | 2-3 | Pages | 87-91 |
| PubMed ID | 7540563 | Mgi Jnum | J:26077 |
| Mgi Id | MGI:73699 | Doi | 10.1016/0014-5793(95)00463-j |
| Citation | Manavalan P, et al. (1995) Sequence homologies between nucleotide binding regions of CFTR and G-proteins suggest structural and functional similarities. FEBS Lett 366(2-3):87-91 |
| abstractText | Sequence homology between the alpha-subunits of G-proteins and other GTP-binding proteins and certain regions within the nucleotide binding domains (NBDs) of cystic fibrosis transmembrane conductance regulator (CFTR) indicates that these protein structures may be similar. A sequence alignment of the NBDs of CFTR and NBDs from other membrane transporters, forms the basis of a structural model. This model predicts that one of the conserved sequences GGQR, within which a number of CF mutations occur, forms part of the nucleotide binding pocket and serves as an ON/OFF conformational switch as observed in GTP binding proteins. Furthermore, based on subtle sequence differences between the first and second NBDs of CFTR and from structure-activity data, we suggest that the nucleotide binding site environments of the two NBDs are different. |
