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Publication : Simultaneous visualization of Q-bands and FISH signals using a novel fluorochrome.

First Author  Kondoh Y Year  1995
Journal  Cytogenet Cell Genet Volume  71
Issue  1 Pages  96-8
PubMed ID  7606937 Mgi Jnum  J:27747
Mgi Id  MGI:75232 Doi  10.1159/000134071
Citation  Kondoh Y, et al. (1995) Simultaneous visualization of Q-bands and FISH signals using a novel fluorochrome. Cytogenet Cell Genet 71(1):96-8
abstractText  We developed a new HNPP-azo dye method for detection of fluorescence in situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester), which reacts enzymatically with alkaline phosphatase and azo dye. The FISH staining method permits simultaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes.
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