First Author | Kondoh Y | Year | 1995 |
Journal | Cytogenet Cell Genet | Volume | 71 |
Issue | 1 | Pages | 96-8 |
PubMed ID | 7606937 | Mgi Jnum | J:27747 |
Mgi Id | MGI:75232 | Doi | 10.1159/000134071 |
Citation | Kondoh Y, et al. (1995) Simultaneous visualization of Q-bands and FISH signals using a novel fluorochrome. Cytogenet Cell Genet 71(1):96-8 |
abstractText | We developed a new HNPP-azo dye method for detection of fluorescence in situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester), which reacts enzymatically with alkaline phosphatase and azo dye. The FISH staining method permits simultaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes. |