| First Author | Mandal M | Year | 1995 |
| Journal | Am J Reprod Immunol | Volume | 33 |
| Issue | 1 | Pages | 60-7 |
| PubMed ID | 7619235 | Mgi Jnum | J:29317 |
| Mgi Id | MGI:76846 | Doi | 10.1111/j.1600-0897.1995.tb01139.x |
| Citation | Mandal M, et al. (1995) Purification and characterization of a pregnancy-associated protein: TJ6s. Am J Reprod Immunol 33(1):60-7 |
| abstractText | PROBLEM: Characterization of the soluble form of a novel protein, TJ6 (TJ6s) with immune suppressive activity from murine fetoplacental units. METHOD: Preferential ammonium sulfate precipitation, gel filtration and ion-exchange chromatography were employed to purify the protein TJ6s from murine fetoplacental units using an anti-peptide antibody as a detection tool. Biological activity of the purified protein was studied in lymphocyte proliferation assays. RESULTS: Purified TJ6s has a M(r) of approximately 18 kDa as evidenced by SDS-PAGE in both reducing and non reducing conditions. It exerted a strong anti-proliferative activity in both anti-CD3 and Con A proliferation lymphocyte proliferation assays but not in a PHA assay, suggesting that the anti-proliferative effects on T cells are exerted only on cells specifically activated directly through T cell receptor complex. CONCLUSION: The results indicate that TJ6s is a novel anti-proliferative protein that has many of the characteristics that are considered necessary for survival of the fetal allograft. |
