| First Author | Riego E | Year | 1995 |
| Journal | Mol Reprod Dev | Volume | 41 |
| Issue | 2 | Pages | 157-66 |
| PubMed ID | 7654369 | Mgi Jnum | J:26126 |
| Mgi Id | MGI:73748 | Doi | 10.1002/mrd.1080410206 |
| Citation | Riego E, et al. (1995) Differential constitutive expression of interferon genes in early mouse embryos. Mol Reprod Dev 41(2):157-66 |
| abstractText | Recent evidence suggests that several processes during mammalian embryogenesis may be regulated by IFNs or IFN-like molecules. With the use of MAPPing, the simultaneous presence of transcripts homologous to IFN-alpha, IFN-beta, IRF-1, and IRF-2 was examined in mouse embryos and in embryonal carcinoma (EC) P19 cells, which are equivalent to epiblast cells of the early postimplantation blastocysts. Transcripts for IFN-alpha, but not for IFN-beta, were detected as maternal transcripts in the ovulated oocyte and persisted over early embryogenesis. IRF-1 transcripts appeared only after the first cell cleavage in the two-cell stage embryo. IRF-2 transcripts were analyzed only in EC P19 cells and were found in both undifferentiated (D-) and differentiated (D+) cells. The IFN-alpha transcripts present in (D-) P19 cells were cloned and the partial cDNA sequences determined. Mu IFN-alpha A and a new Mu IFN-alpha species (Mu IFN-alpha 12) were isolated from (D-) P19 cells. The presence of constitutive IFN-alpha transcripts in early mouse embryos suggests a role for these molecules during embryogenesis. |
