| First Author | Shieh SY | Year | 1995 |
| Journal | J Biol Chem | Volume | 270 |
| Issue | 37 | Pages | 21503-8 |
| PubMed ID | 7665561 | Mgi Jnum | J:28757 |
| Mgi Id | MGI:76299 | Doi | 10.1074/jbc.270.37.21503 |
| Citation | Shieh SY, et al. (1995) Molecular characterization of the rat insulin enhancer-binding complex 3b2. Cloning of a binding factor with putative helicase motifs. J Biol Chem 270(37):21503-8 |
| abstractText | Cell-specific expression of the rat insulin II gene is in part mediated through an element located in the 5'-flanking region. The element, termed RIPE3b (-126 to -101), confers beta-cell-specific expression in conjunction with an adjacent element RIPE3a (-110 to -86). Here we report the characterization of one of the RIPE3b-binding complexes, 3b2. UV cross-linking analysis demonstrated that it is composed of at least three polypeptides: p58, p62, and p110. Furthermore, a cDNA was isolated via expression screening for binding to RIPE3b. Sequence analysis reveals that the encoded protein, designated Rip-1, possessed putative helicase motifs and a potential transcription activation domain. Overexpression of Rip-1 in cells greatly enhances the 3b2 binding complex, suggesting that Rip-1 is involved in the binding of 3b2. |
