| First Author | Suetake I | Year | 1995 |
| Journal | J Biochem | Volume | 118 |
| Issue | 1 | Pages | 244-9 |
| PubMed ID | 8537320 | Mgi Jnum | J:31663 |
| Mgi Id | MGI:79151 | Doi | 10.1093/oxfordjournals.jbchem.a124886 |
| Citation | Suetake I, et al. (1995) A novel DNA binding protein that recognizes the methylated c-Myc binding motif. J Biochem 118(1):244-9 |
| abstractText | We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding motif. Its binding to the methylated c-Myc binding motif was specific, although it also recognized the unmethylated motif weakly. MMBP-3 was further found to recognize only one of two differently hemimethylated forms of the double-stranded c-Myc binding motif. MMBP-3 activity was detected in proliferating C2C12 and C3H/10T1/2 cells, and down regulated when the growth of these cells was inhibited. We propose that MMBP-3 plays a role in regulating the c-Myc function by recognizing the methylation state of the c-Myc binding motif in a growth-dependent manner. |
