| First Author | Wilder JA | Year | 1995 |
| Journal | Int Immunol | Volume | 7 |
| Issue | 4 | Pages | 575-82 |
| PubMed ID | 7547684 | Mgi Jnum | J:25094 |
| Mgi Id | MGI:72807 | Doi | 10.1093/intimm/7.4.575 |
| Citation | Wilder JA, et al. (1995) Regulation of IFN-gamma mRNA production in murine natural killer cells. Int Immunol 7(4):575-82 |
| abstractText | We have previously reported that large, presumably in vivo activated, B cells stimulate murine natural killer (NK) cells to secrete increased levels of IFN-gamma. In order to further understand the mechanism of IFN-gamma induction, we compared the regulation of IFN-gamma mRNA production after stimulation of NK cells with either B lymphocytes or phorbol myristate acetate (PMA)+ionomycin. Here we show that stimulation of NK cells by either stimuli results increase in IFN-gamma mRNA, albeit with different kinetics. Although the induction requires new RNA synthesis, we could not detect increased transcription of the IFN-gamma gene after stimulation. Measurement of the rate of mRNA degradation after IFN-gamma mRNA has accumulated demonstrates that this mRNA is more stable than IFN-gamma mRNA from unstimulated NK cells. Together, these results suggest that the increase in IFN-gamma mRNA and protein in NK cells, stimulated by B cells or PMA+ionomycin, results from stabilization of pre-existing IFN-gamma message. Our results also suggest that induction of the factor which stabilizes the mRNA, although as yet unknown, requires new RNA synthesis. |
