| First Author | Sato K | Year | 1995 |
| Journal | J Neurochem | Volume | 65 |
| Issue | 5 | Pages | 1967-73 |
| PubMed ID | 7595479 | Mgi Jnum | J:29345 |
| Mgi Id | MGI:76870 | Doi | 10.1046/j.1471-4159.1995.65051967.x |
| Citation | Sato K, et al. (1995) Modulation of a recombinant glycine transporter (GLYT1b) by activation of protein kinase C. J Neurochem 65(5):1967-73 |
| abstractText | Treatment of human embryonic kidney cells (HEK 293 cells) expressing the mouse glycine transporter 1 (GLYT1b) with the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) decreased specific [3H]glycine uptake. This down-regulation resulted from a reduction of the maximal transport rate and was blocked by the PKC inhibitors 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7) and staurosporine. The inhibitory effect of PMA treatment was also observed after removing all five predicted phosphorylation sites for PKC in GLYT1b by site-directed mutagenesis. These data indicate that glycine transport by GLYT1b is modulated by PKC activation; however, this regulation may involve indirect phosphorylation mechanisms. |
