| First Author | Hulsebos TJ | Year | 1995 |
| Journal | Genomics | Volume | 28 |
| Issue | 3 | Pages | 543-8 |
| PubMed ID | 7490092 | Mgi Jnum | J:28408 |
| Mgi Id | MGI:76026 | Doi | 10.1006/geno.1995.1186 |
| Citation | Hulsebos TJ, et al. (1995) Identification of the human beta A2 crystallin gene (CRYBA2): localization of the gene on human chromosome 2 and of the homologous gene on mouse chromosome 1. Genomics 28(3):543-8 |
| abstractText | By using primers synthesized on the basis of the bovine beta A2 crystallin gene sequence, we amplified exons 5 and 6 of the human gene (CRYBA2). CRYBA2 was assigned to human chromosome 2 by concordance analysis in human x rodent somatic cell hybrids using the amplified PCR products as probe. Regional localization to 2q34-q36 was established by hybridizing the CRYBA2 probe to microcell and radiation hybrids containing defined fragments of chromosome 2 as the only human contribution. The CRYBA2 probe was also used to localize, by interspecific backcross mapping, the mouse gene (Cryba2) to the central portion of chromosome 1 in a region of known human chromosome 2 homology. Finally, we demonstrate that in both species the beta A2 crystallin gene is linked but separable from the gamma A crystallin gene. The beta A2 crystallin gene is a candidate gene for human and mouse hereditary cataract. |
