| First Author | Römer I | Year | 1995 |
| Journal | Electrophoresis | Volume | 16 |
| Issue | 5 | Pages | 823-30 |
| PubMed ID | 7588571 | Mgi Jnum | J:31104 |
| Mgi Id | MGI:78575 | Doi | 10.1002/elps.11501601137 |
| Citation | Romer I, et al. (1995) A novel strategy to identify maternal and paternal inheritance in the mouse. Electrophoresis 16(5):823-30 |
| abstractText | A novel strategy for identifying proteins which reveal maternal or paternal inheritance in the mouse is presented. Using two-dimensional electrophoresis we investigated protein expression patterns of adult liver and different embryonic and extraembryonic tissue in C57BL/6Crl and in DBA/2Crl mice, as well as in their reciprocal hybrids. We found three groups of protein spots which showed maternal or paternal inheritance of quantitative variations. These proteins were characterized by N-terminal or internal amino acid sequencing, by determination of the amino acid composition, by glycoprotein staining and RNA expression analysis. The three proteins identified were: alpha-enolase, cyclophilin and beta-group hemoglobins. The parental effects observed for alpha-enolase and cyclophilin were found to be due to parent-specific post-translational modifications of these proteins. For the beta-group hemoglobins our results suggested parental effects on the transcriptional level. |
