| First Author | Fitzpatrick DR | Year | 1995 |
| Journal | J Immunol | Volume | 155 |
| Issue | 11 | Pages | 5140-50 |
| PubMed ID | 7594523 | Mgi Jnum | J:29870 |
| Mgi Id | MGI:77394 | Doi | 10.4049/jimmunol.155.11.5140 |
| Citation | Fitzpatrick DR, et al. (1995) Dissociated expression of granulocyte-macrophage CSF and IL-3 in short-term T cell clones from normal mice. J Immunol 155(11):5140-50 |
| abstractText | Granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-3 are generally thought to be produced in a coordinated fashion by all activated T cells. We have examined this premise using quantitative kinetic analyses of the expression of GM-CSF and IL-3 in clonal cultures of mouse T cells for the first two weeks following in vitro stimulation with solid-phase TCR- and accessory molecule-specific mAbs. We demonstrate that 1) differential secretion of GM-CSF and IL-3 is a feature of high proportions of CD8+ and CD4+ T cell clones, for extended periods of time following activation; 2) multiple patterns of expression of these two cytokines can occur, including equivalent, GM-CSF-biased, and IL-3-biased production, and a pattern that switches over a period of days from GM-CSF-biased to IL-3-biased production; 3) the disparate relative levels of secretion are not due to differential consumption of either cytokine; 4) altered T cell activation by addition of CD28 costimulation accelerates both GM-CSF and IL-3 production but biased patterns of expression are retained, and 5) most T cells are not pre-committed to a particular pattern of relative GM-CSF and IL-3 expression; instead, the potential for multiple patterns may persist for several days following in vitro activation. The results indicate that T cells have the potential to display previously unrecognized diversity of expression of GM-CSF and IL-3. |
