| First Author | Tanaka I | Year | 1995 |
| Journal | FEBS Lett | Volume | 376 |
| Issue | 3 | Pages | 146-50 |
| PubMed ID | 7498529 | Mgi Jnum | J:30665 |
| Mgi Id | MGI:78169 | Doi | 10.1016/0014-5793(95)01262-2 |
| Citation | Tanaka I, et al. (1995) Unusual long target duplication by insertion of intracisternal A-particle element in radiation-induced acute myeloid leukemia cells in mouse. FEBS Lett 376(3):146-50 |
| abstractText | Retrotransmission into the IL-3/GM-CSF gene locus by the retrotransposon intracisternal A-particle (IAP) had been observed in distinct tumor cell lines. We analyzed the locus in genomes from 7 different myeloid leukemia cell strains which were originally generated by whole-body X-irradiation of the inbred C3H/He mice at a dose of 3 Gy and maintained by in vivo passage. In one leukemia cell strain out of 7 such cases, RFLP of an allele of the interleukin-3 gene was found. Sequence analysis after cloning from the genomic library showed that a type I delta 2 IAP element was inserted in the region upstream of the IL-3 gene in the head-to-head orientation. This suggests that the locus in myeloid cells is sensitive for integration of IAP elements. Additionally, an unusual long target duplication of 82 bp, 14-fold larger than normal one, was found at the junction of the element. This suggests the possibility of a radiation-induced integration mechanism which is distinct from normal retrotransmission. |
