| First Author | Kapil S | Year | 1995 |
| Journal | Comp Immunol Microbiol Infect Dis | Volume | 18 |
| Issue | 4 | Pages | 245-52 |
| PubMed ID | 8549113 | Mgi Jnum | J:35921 |
| Mgi Id | MGI:83364 | Doi | 10.1016/0147-9571(95)00016-2 |
| Citation | Kapil S (1995) Minute virus of mice (MVM) nucleic acid production in susceptible and resistant strains of mice and F1 hybrids. Comp Immunol Microbiol Infect Dis 18(4):245-52 |
| abstractText | Susceptibility of neonatal mice to the lethal effects of the virulent allotropic variant of minute virus of mice is controlled by host genotype. We quantitated MVM-DNA production in resistant C57BL/6 (B6), susceptible C3H/He (H), and (B6 x H) F1 (F1) hybrid mice. Total DNA extracted from six organs of infected mice on post inoculation days (PID) 3, 5, 7, and 9 was analyzed by dot blot and Southern blot assays. 7 to 100-fold more viral DNA was produced in H mice than in B6 mice on PID 7 depending on the organ and F1 mice phenotypically resembled B6 mice. Southern blot analysis of DNA extracted from infected mice on PID 7 showed a prominent single-stranded band (5 kb, virion DNA) and a faint replicative form (5 kb, double-stranded) in all strains of mice. Total RNA extracted from the organs of B6, H, and F1 mice was analyzed by Northern blot. Viral mRNA production was generally below detection limits. In kidneys, hearts, and intestines of some H mice on PID 7, however, a 1.9 kb transcript was detected, smaller than transcripts for R1, R2 or R3. This study confirms that susceptibility to lethal MVMi infection correlates with upregulation of MVM DNA production, a recessive trait, and perhaps with the production of a novel 1.9 kb transcript. |
