| First Author | Krishnan BR | Year | 1995 |
| Journal | Genomics | Volume | 30 |
| Issue | 1 | Pages | 53-8 |
| PubMed ID | 8595903 | Mgi Jnum | J:29691 |
| Mgi Id | MGI:77216 | Doi | 10.1006/geno.1995.0008 |
| Citation | Krishnan BR, et al. (1995) Feature mapping of the HLA class I region: localization of the POU5F1 and TCF19 genes. Genomics 30(1):53-8 |
| abstractText | The class I region of the human leukocyte antigen (HLA) complex located on chromosome 6p21.3 is gene dense. To define the gene content of the class I region, we are constructing genomic DNA feature maps. Here we report mapping of the POU5F1 and TCF19 genes to an approximately 0.2-Mb region between the HLA-C and the S genes. Localization of these genes was facilitated by subcloning genomic DNA fragments from the 0.2-Mb region into a transposon gamma delta-based vector, selecting transposon-mediated deletions in vivo in Escherichia coli, and sequencing a nested subset chosen for their uniform distribution of deletion endpoints. The POU5F1 and TCF19 genes are located approximately 130 kb telomeric of the HLA-C locus, approximately 0.6 kb apart from each other. Complete sequencing of a 5.5-kb EcoRI fragment containing the TCF19 gene revealed that it is composed of three exons, bounded by consensus splice signals. These experiments illustrate that the transposon-based nested deletion sequencing method provides an easy and efficient approach to feature mapping genomic fragments and to high-resolution analysis of selected subportions. |
