| First Author | Zhao C | Year | 1996 |
| Journal | Exp Hematol | Volume | 24 |
| Issue | 1 | Pages | 54-8 |
| PubMed ID | 8536793 | Mgi Jnum | J:30423 |
| Mgi Id | MGI:77934 | Citation | Zhao C, et al. (1996) Erythropoietin-like activity in vivo of the fusion protein rhIL-6/IL-2 (CH925). Exp Hematol 24(1):54-8 |
| abstractText | CH925 is a novel cytokine of a fusion protein interleukin-6 (IL-6)/IL-2 exhibiting erythropoietin (Epo)-like effects in vivo and ex vivo, in addition to its enhanced effects compared to IL-2 and IL-6 reported by us previously, which indicates its potential clinical use. Our present study was undertaken to determine the Epo-like activity of CH925 in vivo. The reticulocyte response was observed in transfusion-induced polycythemic mice by using flow cytometry with pyronin Y staining. On day 2 after injection of CH925, the average number of reticulocytes was 2.11% in the group given 250 micrograms/kg/d and 1.01% for 100 micrograms/kg/d. The mean fluorescence intensity (MFI) also significantly increased. Longitudinal studies of CH925 were performed on days 2, 4, and 10, and reticulocyte counts increased up to a peak on day 4. Activity of CH925 (100 micrograms/kg/d) corresponds to 1 U of standard rhEpo in our study. |
