| First Author | Osanai T | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 218 |
| Issue | 2 | Pages | 610-5 |
| PubMed ID | 8561804 | Mgi Jnum | J:31526 |
| Mgi Id | MGI:79032 | Doi | 10.1006/bbrc.1996.0108 |
| Citation | Osanai T, et al. (1996) Two families of murine carbohydrate ligands for E-selectin. Biochem Biophys Res Commun 218(2):610-5 |
| abstractText | In search of endogenous oligosaccharide ligands for the endothelial adhesion molecule E-selectin in mouse, glycolipids from tissues and the neutrophilic cell line 32D c13 were tested for E-selectin binding. Kidneys of BALB/c and NMRI mice (but not CBA) and the 32D c13 cells were found to contain minor glycolipid populations that support strongly the binding of murine E-selectin. By chromatogram binding experiments and in situ liquid secondary ion mass spectrometry (LSIMS) with neoglycolipids derived from their endoglycoceramidase-released oligosaccharides, in conjunction with compositional and linkage analyses, one of the glycolipid ligands in kidney was identified as the Le(x)-active extended globo-glycolipid: [formula: see text] Neoglycolipids enriched for the ligand structures were obtained from oligosaccharides released by endo-beta-galactosidase from the 32D c13 cells. By TLC-LSIMS and antibody binding, the main E-selectin binding determinant on these was identified as sialyl-Le(a). |
