| First Author | Pergolizzi R | Year | 1996 |
| Journal | Gene | Volume | 168 |
| Issue | 2 | Pages | 267-70 |
| PubMed ID | 8654957 | Mgi Jnum | J:31776 |
| Mgi Id | MGI:79262 | Doi | 10.1016/0378-1119(95)00741-5 |
| Citation | Pergolizzi R, et al. (1996) Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region. Gene 168(2):267-70 |
| abstractText | To contribute to the isolation of genes within the q-24-qter region of the human X chromosome,we screened three cDNA libraries (human fetal brain, liver and skeletal muscle) with a cosmid clone containing a CpG island previously mapped in the q28 region. A full-length 2.1-kb cDNA clone was isolated (XIB); DNA databank searches revealed identity with an EST fragment (XAP-1), residing between the RCP/GCP and G6PD loci. The XIB coding region (909 bp) showed 44% amino acid (aa) identity to pig DNase I. Several conserved residues have been observed between these two genes including aa in the active site. XIB expressed a single transcript in adult heart and skeletal muscle, whereas, in some fetal tissues, two different-sized transcripts were seen. Zoo blot analysis showed a remarkable cross-species conservation. Expression and sequence of this novel gene are reported. |
