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Publication : Mapping of the porcine urate oxidase and transforming growth factor beta 2 genes by fluorescence in situ hybridization.

First Author  Rettenberger G Year  1996
Journal  Chromosome Res Volume  4
Issue  2 Pages  147-50
PubMed ID  8785609 Mgi Jnum  J:31749
Mgi Id  MGI:79235 Doi  10.1007/BF02259708
Citation  Rettenberger G, et al. (1996) Mapping of the porcine urate oxidase and transforming growth factor beta 2 genes by fluorescence in situ hybridization. Chromosome Res 4(2):147-50
abstractText  We have mapped two genes from human chromosome 1, urate oxidase (UOX) and transforming growth factor beta 2 (TGFB2), by fluorescence in situ hybridization (FISH) in the pig genome. Porcine-specific polymerase chain reaction (PCR) primers for both genes were designed from the porcine cDNA sequence. With the help of these primers yeast artificial chromosome (YAC) clones for UOX and TGFB2 were isolated from a pig YAC library. These DNA probes were used for FISH analysis. TGFB2 was localized to SSC 10p16. With the YAC probe for UOX two porcine chromosome regions 6q26 and 6q32, revealed specific signals. These results, help to refine the comparative mapping data between human and pig.
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