| First Author | Bain G | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 223 |
| Issue | 3 | Pages | 691-4 |
| PubMed ID | 8687458 | Mgi Jnum | J:33873 |
| Mgi Id | MGI:81366 | Doi | 10.1006/bbrc.1996.0957 |
| Citation | Bain G, et al. (1996) Retinoic acid promotes neural and represses mesodermal gene expression in mouse embryonic stem cells in culture. Biochem Biophys Res Commun 223(3):691-4 |
| abstractText | Mouse embryonic stem cells treated with retinoic acid are induced to differentiate into neuron-like cells (Bain et al. (1995) Dev. Biol. 168, 342-357). Here we have examined the expression of a set of neural- and mesoderm-specific genes during this in vitro differentiation process. mRNAs encoding the neural genes Wnt-1, MASH1, the light and medium isoforms of neurofilaments, and the neurotransmitter-synthesizing enzyme glutamic acid decarboxylase are all strongly upregulated by retinoic acid treatment; expression of these genes occurs in a temporal pattern resembling that in the developing brain. In contrast, retinoic acid blocks the expression of the mesodermal genes Brachyury, cardiac actin, and zeta-globin. Thus, retinoic acid exerts both pro-neuronal and anti-mesodermal activities on mouse embryonic stem cells in culture. |
