| First Author | Nielsen KL | Year | 1996 |
| Journal | J Biol Chem | Volume | 271 |
| Issue | 22 | Pages | 12909-12 |
| PubMed ID | 8662686 | Mgi Jnum | J:33242 |
| Mgi Id | MGI:80722 | Doi | 10.1074/jbc.271.22.12909 |
| Citation | Nielsen KL, et al. (1996) Identification of residues in alpha-macroglobulins important for binding to the alpha2-macroglobulin receptor/Low density lipoprotein receptor-related protein. J Biol Chem 271(22):12909-12 |
| abstractText | Variants of the receptor binding domain of both human alpha2-macroglobulin and the corresponding domain of hen egg white ovomacroglobulin have been expressed in Escherichia coli and refolded in vitro. Competition experiments with methylamine-treated alpha2-macroglobulin for binding to the multifunctional alpha2-macroglobulin receptor identify two Lys residues (residues 1370 and 1374 in human alpha2-macroglobulin) spaced by three amino acid residues as crucial for receptor binding. From this result and mutational evidence from other ligands for the alpha2-macroglobulin receptor, a tentative sequence motif for receptor binding is proposed. |
