| First Author | Kieffer TJ | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 224 |
| Issue | 2 | Pages | 522-7 |
| PubMed ID | 8702421 | Mgi Jnum | J:34991 |
| Mgi Id | MGI:82443 | Doi | 10.1006/bbrc.1996.1059 |
| Citation | Kieffer TJ, et al. (1996) Leptin receptors expressed on pancreatic beta-cells. Biochem Biophys Res Commun 224(2):522-7 |
| abstractText | Leptin (Ob protein) is a recently isolated hormone produced by adipocytes and is a powerful regulator of satiety centers in the brain. A defect in either leptin production or transmission of the leptin signal in animal models, i.e. ob/ob and db/db mice, respectively, results in a syndrome of obesity and diabetes which closely resembles that which occurs in humans. Leptin release is regulated in part by nutritional status and its expression in adipose tissue is up-regulated by insulin. Since hyperinsulinemia is a primary defect in ob/ob and db/db mice which manifests early in the disease, we postulated that leptin may also regulate insulin release as part of a adipoinsular' feedback loop. We demonstrate the expression of leptin receptor mRNA in primary rat pancreatic islets and in the insulinoma cell line beta TC-3. Furthermore, we find binding of 125I-leptin to beta TC-3 cells which is significantly displaced by leptin. These findings suggest the possibility that the binding of leptin to its receptor in beta-cells may modulate insulin expression in a negative feedback loop, and thereby may have an anti-obesity effect. |
