| First Author | Erickson LD | Year | 1996 |
| Journal | Int Immunol | Volume | 8 |
| Issue | 7 | Pages | 1121-9 |
| PubMed ID | 8757957 | Mgi Jnum | J:34316 |
| Mgi Id | MGI:81776 | Doi | 10.1093/intimm/8.7.1121 |
| Citation | Erickson LD, et al. (1996) Differential expression of CD22 (Lyb8) on murine B cells. Int Immunol 8(7):1121-9 |
| abstractText | Previous studies have established the distribution, biochemistry and functional attributes of human CD22, a B cell-restricted glycoprotein. Recently, molecular cloning of the murine CD22 equivalent revealed this molecule to be the same as the previously described Lyb8 alloantigen. Using the anti-Lyb8 mAb Cy34.1.2, the present report documents the expression patterns of CD22 within the murine B cell compartment. The results demonstrate that in the bone marrow, murine CD22 is absent on the surface of pro-B cells, pre-B cells and newly emerging IgM+ B cells. CD22 is present at a low density on immature IgMhi B cells and fully expressed on mature recirculating B cells. In the periphery, murine CD22 is expressed at mature levels on all B cell subsets including follicular, marginal zone, B1 and switched B cells. Further studies showed CD22 to be retained on activated murine B cells for extended periods. Finally, in combination with CD23 and heat stable antigen, CD22 can be used to delineate the immature splenic B cells, and distinguish them from follicular and marginal zone cells. Together, the results demonstrate murine CD22 to be a useful pan marker for all mature B cell subsets. |
