| First Author | Ogura A | Year | 1996 |
| Journal | J Assist Reprod Genet | Volume | 13 |
| Issue | 5 | Pages | 431-4 |
| PubMed ID | 8739061 | Mgi Jnum | J:45705 |
| Mgi Id | MGI:1195860 | Doi | 10.1007/BF02066177 |
| Citation | Ogura A, et al. (1996) Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring. J Assist Reprod Genet 13(5):431-4 |
| abstractText | PURPOSE: This study was performed to determine whether frozen-thawed mouse round spermatids can fertilize oocytes and contribute to normal embryo development. METHODS: Freshly collected mouse testicular cells were frozen in PBS containing 7.5% glycerol and 7.5% fetal bovine serum. After thawing and removal of the cryoprotectants, round spermatids were selected and injected individually into mature oocytes which had been previously activated with Sr(2+)-containing Ca(2+)-free medium. RESULTS: After thawing, 75-85% of testicular cells were alive. About 90% of the oocytes were fertilized by intracytoplasmic injection of frozen-thawed round spermatids; 11% (17/150) of embryos transferred to foster mothers developed into normal offspring. CONCLUSIONS: Mouse round spermatids can be cryopreserved for production of normal offspring. |
