| First Author | Dattamajumdar AK | Year | 1996 |
| Journal | Immunogenetics | Volume | 44 |
| Issue | 6 | Pages | 432-40 |
| PubMed ID | 8824154 | Mgi Jnum | J:36505 |
| Mgi Id | MGI:83932 | Doi | 10.1007/BF02602804 |
| Citation | Dattamajumdar AK, et al. (1996) Characterization of the mouse Tcra-V22 gene subfamily. Immunogenetics 44(6):432-40 |
| abstractText | T-cell receptors (Tcrs) of higher organisms play a key role in the specific recognition of self and non-self molecules in the immune system. The large number of Tcr variable (V) genes have been organized into V gene subfamilies according to their sequence similarity at the nucleotide and amino acid level. We cloned and characterized four new members of the Tcra-V22 gene subfamily at the genomic level using a simple and sensitive technique that can rapidly clone members of any multi-member gene family. Sequence analysis reveals that the four Tcra-V22 gene subfamily members have more than 98% sequence similarity in their coding regions, at the nucleotide and amino acid levels. However, the intron between the leader and the coding region varies up to 7% between members of the Tcra-V22 gene subfamily. Comparison of the multi-member Tcra-V22 gene subfamily with other multi-member Tcra-V gene subfamilies (V2, V8, and V11), shows that Tcra-V22 is unique in that it has multiple members with nearly identical amino acid sequence and which are not inherently pseudogenes. Sequence similarity analysis of the Tcra-V22 subfamily with the prototypes of all other Tcra-V subfamilies revealed that the Tcra-V22 subfamily has the closest sequence similarity to that of Tcra-V18 (77% at the nucleotide level and 71% at the amino acid level). |
