| First Author | Neuenschwander S | Year | 1996 |
| Journal | Anim Genet | Volume | 27 |
| Issue | 4 | Pages | 275-8 |
| PubMed ID | 8856925 | Mgi Jnum | J:35519 |
| Mgi Id | MGI:82966 | Doi | 10.1111/j.1365-2052.1996.tb00489.x |
| Citation | Neuenschwander S, et al. (1996) Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids. Anim Genet 27(4):275-8 |
| abstractText | Degenerate primers based on human and mouse obesity gene (OBS) sequencing data were used in the reverse transcriptase-polymerase chain reaction (RT-PCR) of total RNA from pig white adipose tissue. Both strands of the resultant pig- specific 325 bp DNA fragment were sequenced. Comparison of the obtained sequence with known sequences revealed an 86% identity with the human and 84% identity with the mouse OBS cDNA. The OBS gene was physically mapped to pig chromosome 18 by PCR analysis of somatic cell hybrids, using pig-specific primers. This result is consistent with the recent assignment of the human OBS gene to chromosome 7 and the observation made by comparative mapping that by using a human chromosome 7 specific library two segments of conserved synteny were detected on porcine chromosomes 9 and 18. We conclude the border of conserved synteny to be in the 7q31-7q32 region of the human chromosome. |
