| First Author | Zhang J | Year | 1996 |
| Journal | Anal Biochem | Volume | 242 |
| Issue | 1 | Pages | 68-72 |
| PubMed ID | 8923966 | Mgi Jnum | J:36615 |
| Mgi Id | MGI:84042 | Doi | 10.1006/abio.1996.0429 |
| Citation | Zhang J, et al. (1996) A yeast three-hybrid method to clone ternary protein complex components. Anal Biochem 242(1):68-72 |
| abstractText | The yeast two-hybrid system is a powerful technique to detect binary protein interactions. In order to facilitate ternary complex analysis, we have developed the yeast three-hybrid system. Using epidermal growth factor (EGF) receptor, Grb2 and Sos proteins as an example, we demonstrated the use of the three-hybrid system to detect formation of a three-component complex, i.e., the Grb2-mediated interactions between EGF receptor and Sos. We then used the three-hybrid system to screen a library with Gal4-DNA binding domain -EGF receptor and Gal4 activation domain-Sos fusion proteins and isolated a plasmid encoding Grb2. Thus, the three-hybrid system is useful for characterizing and cloning components of ternary protein complexes. |
