| Year | 1996 | Journal | Prostaglandins |
| Volume | 51 | Issue | 4 |
| Pages | 275-306 | PubMed ID | 8966221 |
| Mgi Jnum | J:33954 | Mgi Id | MGI:81434 |
| Citation | Salier JP, et al. (1996) Comparative mapping of the prostaglandin-D-synthase and other lipocalin genes in human and mouse chromosomes. (International Colloquium on beta-trace, Osaka, Japan, November 17, 1995. Abstracts). Prostaglandins 51(4):300 (275-306 Abstr.) |
| abstractText | Full text of Abstract: International Colloquium on Beta-Trace: Hayaishi. COMPARATIVE MAPPING OF THE PROSTAGLANDIN-D-SYNTHASE AND OTHER LIPOCALIN GENES IN HUMAN AND MOUSE CHROMOSOMES. J.-P. SALIER 1, P. CHAN 1, D. SIMON-CHAZOTTES 2, M.G. MATTEI 3 and J.L. GUENET 2. 1 INSERM Unit-78 and I.F.R.M.P., Boisguillaume, France; 2 Unite de Genetique des Mammiferes, Institut Pasteur, Paris, France; 3 INSERM Unit-406, Marseille, France. Glutathione-independent Prostaglandin-D-synthase (PTGDS) is a member of a large set of proven or potential lipophilic ligand carriers designated the lipocalin superfamily. The latter is comprised of such proteins as alpha-1-Microglobulin/bikunin precursor (AMBP), orosomucoid (ORM), placenta- associated endometrial protein (PAEP), Complement C8 gamma chain (C8G), major urinary protein (MUP) and neutrophil gelatinase-associated lipocalin (NGAL). We and others have mapped human lipocalin genes (PTGDS, AMBP, ORM1, ORM2, PAEP, C8G) at or close to the q34 band of human (HSA) chromosome 9 long arm (HSA9q34) and mouse lipocalin genes (Ambp, Orm-1, Mup) to the 4B->C band of mouse (MMU) chromosome 4 (MMU4B->C). As HSA9q34 and MMU4B->C are known to be homologous, we have suggested that the ancestral gene for the lipocalin superfamily arose at the ancestral area from which HSA9q34 and MMU4B->C originate. We have recently refined this lipocalin gene map in human and mouse by localizing the mouse PTGDS-encoding gene (Ptgs-3) and other mouse genes coding for C8G (C8g), NGAL (Lcn2) and PAEP (Paep). Using restriction fragment length polymorphisms and linkage analyses in C57BL/6PasxMus spretus interspecific crosses, we have mapped these four genes to the proximal (Ptgs3, C8g, Lcn2) or distal part (Paep) of MMU2. MMU2 carries a counterpart of HSA9q34, as MMU4 does. Therefore, the present-day HSA9q34 area is likely to reflect the ancestral chromosomal area where a lipocalin gene cluster arose by regional duplications prior to human/mouse divergence. The two sets of lipocalin genes at MMU4 and MMU2 indicate that an evolutionary breakpoint within the mouse lipocalin gene cluster followed the human/mouse divergence. Accordingly, we predicted that a lipocalin gene (Lcn2) that maps to MMU2 should map to HSA9q34, which was ultimately verified by in situ hybridization. Prostaglandins 1996:51, April |
